Effect of Olive Leaf Extract inInhibition of Renal Ischemia- Reperfusion Injuries in Rat

Introduction: Renal Ischemia Reperfusion is considered to be one of the causes of acute renal failure (AKI). In addition, renal ischemia is an important incident usually occurs due to renal transplantion, repair of suprarenal aneurysm, hemorrhagic shock. A recent study reported that reactive oxygen species (ROS) are important mediators of AKI. It seems essential to use antioxidant to decrease the effect of free radicals. The olive leaf extract (OLE) has an abundant antioxidant characteristic The purpose of this study was to investigate the effects of olive leaf extract on the control of renal injuries caused by renal Ischemia-Reperfusion in an animal model. Methods: forty male Sprague-dawley rats were randomly divided into 5 equal groups: control, I/R+ 0.5 ml water by gavages, I/R+OLE in 3 different doses ( 25mg/kg, 50mg/kg, 100mg/ kg) once daily for 2 week, prior to I/R. The right and left pedicles clamped for 45 minutes (ischemia), followed by 24 hours of reperfusion. Serum BUN and Creatinine were measured, and serum malondialdehyde(MDA) was determined, renal histologically parameters such as tubular necrosis, eosinophilic casts were studied semi quantitatively and tubular dilatation was measured by motic image plus 2 software, and renal antioxidant enzyme activity such as Glutathione was assessed. Results: I/R group significantly increased serum Cr and BUN, serum MDA, and tubular necrosis, casts, tubular dilatation, and significantly decreased Glutathione compared with the control group. Pretreatment with OLE+I/R in 3 different doses significantly decreased tubular dilatation, tubular necrosis and casts, serum MDA, and increased Glutathione comparedwith the I/R group. But only 4 group significantly decreased serum Cr and BUN compared with the I/R group Conclusion: The findings showed that two week pretreatment of rats with OLE by gavages in its three doses (25mg/kg, 50mg/kg and 100mg/kg) can ameliorate renal injuries compared with I/R group. In order to consider the effects of all antioxidant of OLE in decreasing caused by I/R further researches recommended.